Research Associate, Engineered Cells (Swing Shift)
Synthego is searching for a Research Associate to join our Engineered Cells Operations team and help create the world’s first fully automated Genome Engineering lab.
This position has shift options available:
- Tuesday through Saturday; 2:30PM-11PM
- Sunday through Thursday; 2:30PM-11PM
- Tuesday through Saturday; 6:30AM- 3PM
What You'll Do
- Engineer immortalized and/or hiPSC cells using CRISPR-Cas9, ultimately generating a pool or clonal population
- Perform daily cell culture tasks including feeds, expands, and cryopreservation
- Operate laboratory automation (liquid handling systems, clonal expansion systems)
- Perform daily operational checks, calibrations, and maintenance as required
- Extract genomic DNA, perform PCR assembly, and analyze samples using gel electrophoresis
- Review Sanger Sequencing data and determine next steps in the manufacturing process
- Receive and prepare laboratory reagents; update LIMS during the manufacturing process
- Maintain laboratory inventory, equipment, record-keeping and administrative tasks for cell line maintenance and related projects
- Draft and revise manufacturing standard operating procedures (SOPs) and work instructions (WIs)
- Perform laboratory cleaning and maintenance as per rotation schedule
- Acquire and analyze microscopy images
- Participate in company meetings and present data and project plans
- Work closely with Process Engineering to define user requirements for new technologies and to execute performance qualification (PQ) experiments
About You
- BS in molecular and cell biology and biology or related field
- Minimum of 1 year of cell culture experience
- Experience working with a variety of immortalized lines
- Excellent aseptic technique and understanding of contamination control
- Strong organizational, time management, and interpersonal skills
- Excellent verbal and written communication skills
- Ability to work cross functionally with various personas
- Team player who thrives in a fast paced environment
Nice to Haves
- Experience with transfections and genetic engineering of mammalian cells
- Direct experience handling induced pluripotent stem cells (iPSC) or primary cells
- Experience with flow cytometry
- Familiarity with Laboratory Information Management Systems (LIMS)
Company Benefits & Perks
- Competitive salary
- Generous equity at an early stage company
- Robust medical, dental and vision benefits
- 401k Plan
- Fully stocked kitchen and snacks
- Free catered lunches and dinners
- Onsite gym
- Daily shuttle bus to & from San Francisco